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Lab Module 11 - Kirby Bauer method

Lab Module 11 - Kirby Bauer method

Q After reviewing the Lab Module 11 – Kirby-Bauer Antibiotic Susceptibility Testing Introduction and Background document and the supplemental lab videos, complete the lab report below. Use the reference chart on the susceptibilities of antibiotics on page 2. Indicate for each strain below based on measurements of the diameter of the zone of inhibition, if it is susceptible (S), intermediate (I) or resistant (R) by placing an X in the appropriate box. Staphylococcus aureus 1.1. Based on your results from above, which is the best antibiotic for each bacterium tested 2. 2. Based on your results, which bacterium demonstrates the greatest resistance overall to the antibiotics used in this exercise? What features of the bacterium likely contribute to the resistance?

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1. Staphlococcus aureus – Ampicillin Pseudomonas aerogens – Tetracycline Serratia marcescens – Chloramphenicol 2. Pseudomonas aeruginosa is greater resistant to the antibiotics . The major mechanism of Pseudomonas aeruginosa to counter antibiotic attack can be classified into intrinsic, acquired and adaptive resistance.